Type of Enzyme
Applications
-
Nick translation
-
cDNA synthesis
In the Public Domain since
January 3, 2025
Application Features
Nick Translation, DNA replication and repair function
It has an inherent 3′ to 5′ and 5′ to 3′ exonuclease activity
Original Paper
Lehman, I.R., Bessman, M.J., Simms, E.S. and Kornberg, A., 1958. Enzymatic synthesis of deoxyribonucleic acid I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. Journal of Biological Chemistry, 233(1), pp.163-170.
- DNA Sequence (optimised & domesticated)