Type of Enzyme
Applications
In the Public Domain since
May 11, 2010
Application Features
Thermococcus litoralis DNA polymerase
PCR, Sequencing
No 3´→ 5´ proofreading exonuclease activity
2X higher fidelity than Taq
Difficult templates: ideal for GC-rich or looped sequence
High thermostability: half-life of 6.7 hours at 95°C for maximum activity during PCR
Original Paper
Kong, H., Kucera, R.B. and Jack, W.E., 1993. Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. Journal of Biological Chemistry, 268(3), pp.1965-1975.
- DNA Sequence (optimised & domesticated)