Type of Enzyme
Applications
In the Public Domain since
September 30, 2019
Application Features
9°N-7 (D141A / E143A / A485L)
Applications:
Enhanced ability to incorporate modified nucleotides such as 3´-amino-dNTPs, 3´-azido-dNTPs and other
nucleotide analogs modified at the 3´ ribose position.
Incorporation of nucleotide analogs with 3´-OH substitutions, DNA labeling with 3´-amino-ddNTP
Can be used in medical diagnostics.
DNA sequencing by partial ribosubstitution
DNA sequencing using dideoxy or acyclo chain terminators
SNP analysis with dideoxy or acyclo chain terminators
Original Paper
Gardner, A.F. and Jack, W.E., 2002. Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases. Nucleic Acids Research, 30(2), pp.605-613.
https://doi.org/10.1093/nar/30.2.605
- DNA Sequence (optimised & domesticated)