Type of Enzyme
Applications
In the Public Domain since
June 22, 2007
Application Features
T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The high polymerization rate of the enzyme makes long incubations unnecessary.
Radialabelled RNA probe preparation, RNA generation and studies of RNA structure, processing and catalysis, Expression control via anti-sense RNA
Using in the dideoxy sequencing method.
Original Paper
Mark, D. F., & Richardson, C. C. (1976). Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences, 73(3), 780-784.
- DNA Sequence (optimised & domesticated)