Type of Enzyme
Applications
In the Public Domain since
May 11, 2025
Application Features
Bsu DNA Polymerase I, Large Fragment retains the 5´→ 3´ polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5´→ 3´ exonuclease domain. This large fragment naturally lacks 3´→ 5´ exonuclease activity.
– Random primer labeling
– Second strand cDNA synthesis
– Single dA tailing
– Strand displacement DNA synthesis
Original Paper
Okazaki, T. and Kornberg, A., 1964. Enzymatic synthesis of deoxyribonucleic acid XV. Purification and properties of a polymerase from Bacillus subtilis. Journal of Biological Chemistry, 239(1), pp.259-268.
- DNA Sequence (optimised & domesticated)