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Bsu DNA Polymerase I, Large Fragment

Type of Enzyme

  • DNA Polymerase

Applications

  • Strand displacement DNA synthesis

In the Public Domain since

June 7, 2023

Application Features

Bsu DNA Polymerase I, Large Fragment retains the 5´→ 3´ polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5´→ 3´ exonuclease domain. This large fragment naturally lacks 3´→ 5´ exonuclease activity.
– Random primer labeling
– Second strand cDNA synthesis
– Single dA tailing
– Strand displacement DNA synthesis

Original Paper

Okazaki, T. and Kornberg, A., 1964. Enzymatic synthesis of deoxyribonucleic acid XV. Purification and properties of a polymerase from Bacillus subtilis. Journal of Biological Chemistry, 239(1), pp.259-268.

  • DNA Sequence (optimised & domesticated)

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