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Type of Enzyme
Applications
In the Public Domain since
June 7, 2023
Application Features
Labeling
It is used in flourescent labelling for microarray, used in dA and dT tailling, an important in the process of ligating DNA adapters to DNA fragments, frequently used in prepararing DNA libraries for Next-Gen sequencing.
The exo-klenow polymerase is a mutant polymerase from the Klenow DNA polymerase large fragment that lacks the 5′-3′ polymerase activity and the 3′-5′ exonuclease activity.
Original Paper
Freemont, P.S., Ollis, D.L., Steitz, T.A. and Joyce, C.M., 1986. A domain of the Klenow fragment of Escherichia coli DNA polymerase I has polymerase but no exonuclease activity. Proteins: Structure, Function, and Bioinformatics, 1(1), pp.66-73.
- DNA Sequence (optimised & domesticated)