Type of Enzyme
In the Public Domain since
July 25, 2016
DNA polymerase comprising substantially the same amino acid sequence as that of Thermus aquaticus DNA polymerase, excluding the N-terminal 280 amino acid residues of Thermus aquaticus DNA polymerase. Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase. Used in combination with a very low level of a thermostable DNA polymerase exhibiting a 3′-exonuclease activity (Pfu, Vent, or Deep Vent) for long PCR.
Barnes, W.M., 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proceedings of the National Academy of Sciences, 91(6), pp.2216-2220. https://doi.org/10.1073/pnas.91.6.2216