Type of Enzyme
Applications
In the Public Domain since
July 25, 2016
Application Features
DNA polymerase comprising substantially the same amino acid sequence as that of Thermus aquaticus DNA polymerase, excluding the N-terminal 280 amino acid residues of Thermus aquaticus DNA polymerase. Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase. Used in combination with a very low level of a thermostable DNA polymerase exhibiting a 3′-exonuclease activity (Pfu, Vent, or Deep Vent) for long PCR.
Original Paper
Barnes, W.M., 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proceedings of the National Academy of Sciences, 91(6), pp.2216-2220. https://doi.org/10.1073/pnas.91.6.2216
- DNA Sequence (optimised & domesticated)