Type of Enzyme
Applications
In the Public Domain since
May 11, 2020
Application Features
High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications. Generates blunt-ended PCR product.
Lacks 3′-5′ exonuclease activity
Original Paper
Nishioka, M., Mizuguchi, H., Fujiwara, S., Komatsubara, S., Kitabayashi, M., Uemura, H., Takagi, M. and Imanaka, T., 2001. Long and accurate PCR with a mixture of KOD DNA polymerase and its exonuclease deficient mutant enzyme. Journal of biotechnology, 88(2), pp.141-149. https://doi.org/10.1016/S0168-1656(01)00275-9
- DNA Sequence (optimised & domesticated)