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Type of Enzyme
Applications
In the Public Domain since
June 7, 2023
Application Features
Originally isolated from the thermophilic archaebacteria Pyrococcus woesei. Pwo DNA Polymerase exhibits high levels of 3‘→5‘ exonuclease (proofreading) activity and 5‘→3‘ processivity for use when high levels of PCR accuracy are essential. It also exhibits very high thermal stability and will generate PCR products without overhanging ends, making it suitable for ‘blunt end’ cloning experiments.
Original Paper
Frey, B. and Suppmann, B., 1995. Demonstration of the Expand PCR system’s greater fidelity and higher yields with a lacI-based PCR fidelity assay. Biochemica, 2, pp.8-9.
Ghasemi, A., Salmanian, A.H., Sadeghifard, N., Salarian, A.A. and Gholi, M.K., 2011. Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei. Iranian journal of microbiology, 3(3), p.118.
- DNA Sequence (optimised & domesticated)