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Pyrococcus Sp. Heat-Stable (exo–) DNA Polymerase

Type of Enzyme

  • DNA Polymerase

Applications

  • PCR

In the Public Domain since

February 16, 2014

Application Features

PCR (long)
Has a fidelity of 5x higher than taq polymerase, extremely high thermostability (half-life of 23hr for 95 deg celcius)
Ideal fo G-C rich or looped sequences

Original Paper

HUANG, H. and KEOHAVONG, P., 1996. Fidelity and predominant mutations produced by deep vent wild-type and exonuclease-deficient DNA polymerases during in vitro DNA amplification. DNA and cell biology, 15(7), pp.589-594.

  • DNA Sequence (optimised & domesticated)

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