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Type of Enzyme
Applications
In the Public Domain since
August 12, 2011
Application Features
PCR (high-fidelity)
Has a fidelity of 5x higher than taq polymerase, extremely high thermostability (half-life of 23hr for 95 deg celcius)
Ideal for G-C rich or looped sequences.
Original Paper
Xu, M.Q., Southworth, M.W., Mersha, F.B., Hornstra, L.J. and Perler, F.B., 1993. In vitro protein splicing of purified precursor and the identification of a branched intermediate. Cell, 75(7), pp.1371-1377.
- DNA Sequence (optimised & domesticated)