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Type of Enzyme
Applications
In the Public Domain since
July 3, 2009
Application Features
PCR (routine), standard testing, screening and throughput testing
Lacks 3′-5′ exonuclease activity and proofreading ability.
5’→3′ flap endonuclease degrades displaced strand.
Original Paper
Chien A, Edgar DB, Trela JM (1976). “Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus”. J. Bacteriol. 127 (3): 1550–7. PMC 232952 . PMID 8432
- DNA Sequence (optimised & domesticated)