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Type of Enzyme
Applications
In the Public Domain since
June 21, 2011
Application Features
Thermococcus litoralis DNA polymerase
PCR (routine, high-fidelity)
Possesses 3´→ 5´ proofreading exonuclease activity
5X higher fidelity than Taq
Difficult templates: ideal for GC-rich or looped sequence
High thermostability: half-life of 6.7 hours at 95°C for maximum activity during PCR
Original Paper
Mattila, P., Korpela, J., Tenkanen, T. and Pitkämem, K., 1991. Fidelity of DNA synthesis by the Thermococcus litoralis DNA polymerase—an extremely heat stable enzyme with proofreading activity. Nucleic acids research, 19(18), pp.4967-4973.
- DNA Sequence (optimised & domesticated)