Tth DNA polymerase

Type of Enzyme

DNA Polymerase

Applications

Labelling
PCR

In the Public Domain since

August 4, 2018

Application Features

• Tth DNA Polymerase activity is resistant to prolonged incubations at high temperatures (95 °C), and can therefore be used for PCR amplification.
• Tth DNA Polymerase can be used to amplify DNA fragments by the polymerase chain reaction (PCR).
• Since this enzyme also accepts modified deoxyribonucleotides as substrates, it can be used to label DNA fragments with either radiolabeled nucleotides, digoxigenin, or biotin.
• The intrinsic Mn-dependent RT activity allows the enzyme to efficiently transcribe RNA targets into cDNA.
• Tth DNA Polymerase has been used for real time PCR.

Original Paper

RÜTTIMANN, C., COTORÁS, M., ZALDÍÅ, J. and VICUÑA, R., 1985. DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB‐8. European journal of biochemistry, 149(1), pp.41-46.https://doi.org/10.1111/j.1432-1033.1985.tb08890.x

Myers, T.W. and Gelfand, D.H., 1991. Reverse transcription and DNA amplification by a Thermus thermophilus DNA polymerase. Biochemistry, 30(31), pp.7661-7666. DOI: 10.1021/bi00245a001

Auer, T., Landre, P.A. and Myers, T.W., 1995. Properties of the 5′ – 3’Exonuclease/Ribonuclease H Activity of Thermus thermophilus DNA Polymerase. Biochemistry, 34(15), pp.4994-5002. DOI: 10.1021/bi00015a010