Bst DNA Polymerase, Full Length

Type of Enzyme

DNA Polymerase

Applications

Isothermal DNA Amplification

In the Public Domain since

April 12, 2011

Application Features

Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5′ → 3′ polymerase and double-strand specific 5′ → 3′ exonuclease activity, but lacks the 3′ → 5′ exonuclease activity.

Used for: Isothermal DNA amplification, primer extension

Original Paper

Stenesh, J. and Roe, B.A., 1972. DNA polymerase from mesophilic and thermophilic bacteria: I. Purification and properties of DNA polymerase from Bacillus licheniformis and Bacillus stearothermophilus. Biochimica et Biophysica Acta (BBA)-Nucleic Acids and Protein Synthesis, 272(2), pp.156-166.

Kaboev, O.K., Luchkina, L.A., Akhmedov, A.T. and Bekker, M.L., 1981. Purification and properties of deoxyribonucleic acid polymerase from Bacillus stearothermophilus. Journal of bacteriology, 145(1), pp.21-26.