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Bst DNA Polymerase, Full Length

Type of Enzyme

  • DNA Polymerase

Applications

  • Isothermal DNA Amplification

In the Public Domain since

April 12, 2011

Application Features

Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5′ → 3′ polymerase and double-strand specific 5′ → 3′ exonuclease activity, but lacks the 3′ → 5′ exonuclease activity.

Used for: Isothermal DNA amplification, primer extension

Original Paper

Stenesh, J. and Roe, B.A., 1972. DNA polymerase from mesophilic and thermophilic bacteria: I. Purification and properties of DNA polymerase from Bacillus licheniformis and Bacillus stearothermophilus. Biochimica et Biophysica Acta (BBA)-Nucleic Acids and Protein Synthesis, 272(2), pp.156-166.

Kaboev, O.K., Luchkina, L.A., Akhmedov, A.T. and Bekker, M.L., 1981. Purification and properties of deoxyribonucleic acid polymerase from Bacillus stearothermophilus. Journal of bacteriology, 145(1), pp.21-26.

  • DNA Sequence (optimised & domesticated)

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