Type of Enzyme
Applications
In the Public Domain since
July 30, 2016
Application Features
Retain 5´ → 3´ polymerase activity from full length Bst DNA Polymerase, while lacking 5´ →3´ exonuclease activity.
Suitable for applications requiring thermophilic strand displacement.
Used for: Loop-Mediated Isothermal Amplification (LAMP), DNA sequencing through high GC regions, rapid sequencing from nanogram amounts of DNA template.
The enzyme can be used in the standard sanger one step or two step protocol which separates the labelling reaction from the enlongation termination reaction. The enzyme can be used in the double stranded sequencing.
Original Paper
McClary, J., Ye, S. Y., Hong, G. F., & Witney, F. (1991). Sequencing with the large fragment of DNA polymerase I from Bacillus stearothermophilus. DNA Sequence, 1(3), 173-180.
Aliotta, J. M., Pelletier, J. J., Ware, J. L., Moran, L. S., Benner, J. S., & Kong, H. (1996). Thermostable Bst DNA polymerase I lacks a 3′→ 5′ proofreading exonuclease activity. Genetic analysis: biomolecular engineering, 12(5-6), 185-195.
- DNA Sequence (optimised & domesticated)