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Type of Enzyme
Applications
In the Public Domain since
July 29, 2006
Application Features
Degradation of linear ssDNA, linear dsDNA and nicked plasmid DNA while preserving supercoiled plasmid DNA
Used in Gibson Assembly® method
Original Paper
Chatterjeea, D. K., Fujimurab, R. K., Campbella, J. H., & Gerarda, G. F. (1991). Cloning and overexpression of the gene encoding bacteriophage T5 DNA polymerase. Gene, 97(1), 13-19.
- DNA Sequence (optimised & domesticated)